LeviSelect Immune Cell Kits

Uncover the role of immune cells in disease biology

The understanding of the immune system is directly tied to current and future therapeutic approaches regarding human health.  The inherent complexity of the immune system, and the critical role it plays in disease pathology, presents scientists with both technical and analytical hurdles that require optimized sample preparation.

Levitation Technology™ on the LeviCell® platform provides a streamlined workflow to obtain untouched immune cells for immunology, oncology, immuno-oncology and drug discovery applications.

Levitate untouched viable cells of interest with LeviSelect

LeviSelect™ Depletion and Enrichment Kits provide a fast and efficient method to deplete a specific cell type away from a mixture of cells or remove several unwanted cell populations. Enrich for untouched viable specific cells of interest (negative selection) while unwanted cells remain in the LeviCell cartridge. In both kit types, the cells of interest remain label-free, ensuring cells are not perturbed.

Observe cell-specific enrichment in
real-time

Experience the enrichment of viable cell-specific cells during the sample processing step like never before. Levitation Technology allows you to confidently proceed with your project knowing that unwanted cell depletion has occurred – see it in real time! 

By using fluorescence labeling, though not required, cells can be visualized as they are depleted away from cells of interest during levitation. In this video, see the Calcein AM (green) stained cells move quickly down to the bottom of the separation channel leaving only the CellTracker™ Red stained cells levitating in the top channel.

LeviSelect Immune Cell Enrichment Kits 

LeviSelect Immune Kits support cell-specific of the immune subpopulations required for downstream cell analysis or further subsetting. You can enrich hematopoietic populations quickly without lengthy workflows or potential cell activation.

The LeviSelect Immune Cell Enrichment Kit portfolio is continually expanding. Inquire to learn about new kits that are available for early access or let us know what cell populations you are interested in isolating.

Human PBMCs - hCD4 T Cell Enrichment

LeviSelect CD45 Depletion Kits

For oncology or immuno-oncology research, the LeviSelect CD45 Depletion Kits provide a highly effective method for enriching pure tumor cells enabling characterization for drug discovery and biomarker identification. Human or mouse CD45+ cells are depleted from a mixture of cells in parallel with viable cell enrichment on the LeviCell system. CD45+ cells are retained in the LeviCell cartridge, while CD45- cells of interest remain label-free and are collected for further downstream analysis.

Human Colorectal DTCs - hCD45 depletion

Ordering info

Are you ready to upgrade your sample preparation and increase data reliability?

LeviSelect kits

LeviSelect kits can be purchased directly through your local Account Manager.

Limited supplies of new early access kits are now released. Reserve your kit here.

Depletion Kits

Kit size: 10 rxns/kit

Description:
Depletion of human CD45+ cells from a mixture of cells with simultaneous viable cell enrichment using the LeviCell System.  Human CD45+ cells are retained in the LeviCell cartridge, while the human CD45- cells of interest remain label-free and are collected for further downstream analysis.

  • Includes Human anti-CD45 Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Kit size: 10 rxns / kit

Description:
Depletion of mouse CD45+ cells from a mixture of cells with simultaneous viable cell enrichment using the LeviCell System.  Mouse CD45+ cells are retained in the LeviCell cartridge, while the mouse CD45- cells of interest remain label-free and are collected for further downstream analysis.

  • Includes Mouse anti-CD45 Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:

Enrichment Kits

Kit size: 10 rxns / kit

Description:
Enrich label-free human CD3 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human CD3 T cells can be used for various downstream applications.  Non-CD3 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Description:
Enrich label-free human CD4 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human CD4 T cells can be used for various downstream applications.  Non-CD4 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Kit size: 10 rxns / kit

Description:
Enrich label-free human CD8 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human CD8 T cells can be used for various downstream applications.  Non-CD8 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Kit size: 10 rxns / kit

Description:
Enrich label-free human Pan B cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human Pan B Cells can be used for various downstream applications.  Non-B cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents
Kit size: 10 rxns / kit

Description:
Enrich label-free human pan monocytes from peripheral blood mononuclear cell (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human pan monocytes can be used for various downstream applications. Non-monocytes are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing step required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Kit size: 10 rxns / kit

Description:
Enrich label-free mouse Pan B cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse Pan B Cells can be used for various downstream applications.  Non-B cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Kit size: 10 rxns / kit

Description:
Enrich label-free mouse CD3 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse CD3 T cells can be used for various downstream applications.  Non-CD3 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Kit size: 10 rxns / kit

Description:
Enrich label-free mouse CD4 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse CD4 T cells can be used for various downstream applications.  Non-CD4 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Kit size: 10 rxns / kit

Description:
Enrich label-free mouse CD8 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse CD8 T cells can be used for various downstream applications.  Non-CD8 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Kit size: 10 rxns / kit

Description:
Enrich label-free mouse monocytes from a peripheral blood mononuclear (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse monocytes can be used for various downstream applications. Non-monocytes are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing step required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Kit size: 10 rxns / kit

Description:
Enrich label-free mouse NK cells from a peripheral blood mononuclear cell (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse NK cells can be used for various downstream applications. Non-NK cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing step required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents: