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LeviSelectTM

Targeted and viable cell enrichment in a single step

LeviSelect Depletion and Enrichment Kits

LeviSelect Kits provide a fast and efficient method to deplete a specific cell type away from a mixture of cells or remove several cell-specific populations to enrich for a target cell of interest (negative selection). In both kit types, the target cell of interest remains label-free, ensuring cells are not perturbed.

Obtain gentle enrichment of viable target-specific populations, which remain untouched within the LeviCell cartridge. Now, you can achieve dying or dead cell removal—plus removal of debris—in a single LeviCell run.

LeviSelect Immune Kits support target selection of the immune subpopulations required for downstream cell analysis or further subsetting. You can enrich hematopoietic populations quickly without lengthy workflows or potential cell activation.
 

      • CD3 Enrichment
      • CD4 Enrichment
      • CD8 Enrichment
      • Pan B Enrichment
      • Pan Monocyte Enrichment
      • NK Cell Enrichment
For oncology or immuno-oncology research, the LeviSelect CD45 Depletion Kits provide a highly effective method for enriching pure tumor cells enabling characterization for drug discovery and biomarker identification. Human or mouse CD45+ cells are depleted from a mixture of cells in parallel with viable cell enrichment on the LeviCell system. CD45+ cells are retained in the LeviCell cartridge, while CD45- cells of interest remain label-free and are collected for further downstream analysis.
 

      • Includes Human or Mouse anti-CD45 Nanospheres, Buffer, and Protocol
      • No centrifugation or washing steps required
      • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Targeted cell enrichment. Only with the LeviCell system.

Target Specific

  • Enrich specific cells of interest
  • Integrated solution combined with levitation technologies
  • Gentle, efficient, and fast enrichment

Untouched

  • Cells of interest are free from labels
  • Low pressure, minimizing cell stress
  • Limited cell activation and expression profile distortion

Simplified Workflow

  • Shortened incubation time
  • Remove the need for centrifugation
  • No additional wash steps or lengthy processing time

How It Works

LeviSelect products combine Levitation Technology with targeted selection of specific cell types. Targeted and viable cell enrichment are performed simultaneously on the LeviCell system. By combining the natural density and magnetic susceptibility of cells within a paramagnetic compound with the addition of antibodies conjugated to nanospheres of different densities, cells can levitate at different heights. This process allows the separation of specific cells of interest from unwanted cells during a single LeviCell run.

Identifying and enriching cells of interest from mixed cell populations is fundamental to studying disease models and basic cell biology. Levitation Technology provides the gentlest cell enrichment method, preserving target-specific cells of interest unbiasedly.

Target Selection Workflow

Enabling target selection on the LeviCell system is just as easy as viable cell enrichment. Prepare your sample by adding Levitation Agent and LeviSelect reagents. Enrich for cells of interest with the LeviCell system. Collect viable cells for downstream applications.

How To Order

LeviSelect kits can be purchased directly through your local Account Manager. Submit your request here.

Limited supplies of 6 new early access kits are now released. Reserve your kit here.

      • LeviSelect Human Pan Monocyte Enrichment Kit
      • LeviSelect Mouse CD3 T Cell Enrichment Kit
      • LeviSelect Mouse CD4 T Cell Enrichment Kit
      • LeviSelect Mouse CD8 T Cell Enrichment Kit
      • LeviSelect Mouse Monocyte Enrichment Kit
      • LeviSelect Mouse NK Cell Enrichment Kit
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Depletion Kits

LeviSelect Human CD45+ Depletion Kit, PN 1004001

Kit size: 10 rxns/kit

Description:

Depletion of human CD45+ cells from a mixture of cells with simultaneous viable cell enrichment using the LeviCell System.  Human CD45+ cells are retained in the LeviCell cartridge, while the human CD45- cells of interest remain label-free and are collected for further downstream analysis.

  • Includes Human anti-CD45 Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:

LeviSelect Mouse CD45+ Depletion Kit, PN 1004003

Kit size: 10 rxns / kit

Description:

Depletion of mouse CD45+ cells from a mixture of cells with simultaneous viable cell enrichment using the LeviCell System.  Mouse CD45+ cells are retained in the LeviCell cartridge, while the mouse CD45- cells of interest remain label-free and are collected for further downstream analysis.

  • Includes Mouse anti-CD45 Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:

Enrichment Kits

LeviSelect Human CD3 T Cell Enrichment Kit, PN 1004005
Kit size: 10 rxns / kit

Description:

Enrich label-free human CD3 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human CD3 T cells can be used for various downstream applications.  Non-CD3 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:

LeviSelect Human CD4 T Cell Enrichment Kit, PN 1004007
Description:

Enrich label-free human CD4 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human CD4 T cells can be used for various downstream applications.  Non-CD4 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:

LeviSelect Human CD8 T Cell Enrichment Kit, PN 1004009

Kit size: 10 rxns / kit

Description:

Enrich label-free human CD8 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human CD8 T cells can be used for various downstream applications.  Non-CD8 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:

LeviSelect Human Pan B Cell Enrichment Kit, PN 1004011
Kit size: 10 rxns / kit

Description:

Enrich label-free human Pan B cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human Pan B Cells can be used for various downstream applications.  Non-B cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents

LeviSelect Human Pan Monocyte Enrichment Kit, PN 1004027 -
Kit size: 10 rxns / kit

Description:

Enrich label-free human pan monocytes from peripheral blood mononuclear cell (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human pan monocytes can be used for various downstream applications. Non-monocytes are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing step required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:

LeviSelect Mouse Pan B Cell Enrichment Kit A, PN 1004019
Kit size: 10 rxns / kit

Description:

Enrich label-free mouse Pan B cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse Pan B Cells can be used for various downstream applications.  Non-B cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:

LeviSelect Mouse CD3 T Cell Enrichment Kit, PN 1004013 -
Kit size: 10 rxns / kit

Description:

Enrich label-free mouse CD3 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse CD3 T cells can be used for various downstream applications.  Non-CD3 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:

LeviSelect Mouse CD4 T Cell Enrichment Kit, PN 1004015 -
Kit size: 10 rxns / kit

Description:

Enrich label-free mouse CD4 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse CD4 T cells can be used for various downstream applications.  Non-CD4 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:

LeviSelect Mouse CD8 T Cell Enrichment Kit, PN 1004017 -
Kit size: 10 rxns / kit

Description:

Enrich label-free mouse CD8 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse CD8 T cells can be used for various downstream applications.  Non-CD8 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:

LeviSelect Mouse Monocyte Enrichment Kit, PN 1004029 -
Kit size: 10 rxns / kit

Description:

Enrich label-free mouse monocytes from a peripheral blood mononuclear (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse monocytes can be used for various downstream applications. Non-monocytes are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing step required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:

LeviSelect Mouse NK Cell Enrichment Kit, PN 1004033 -
Kit size: 10 rxns / kit

Description:

Enrich label-free mouse NK cells from a peripheral blood mononuclear cell (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse NK cells can be used for various downstream applications. Non-NK cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing step required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents: