New Partnership: LevitasBio and Cytiva

LeviSelect

Viable cell-specific enrichment

Levitate untouched viable cells of interest

The LeviSelect™ Kits that are run on the LeviCell® platform enrich for specific cell types of interest and maintain them as untouched, while simultaneously depleting unwanted cells. As with every LeviCell run, removal of debris, dead or dying cells happens concurrently.

Bridge complex sample types and successful experiments with Levitation Technology and LeviSelect Kits.

LeviSelect Kits

LeviSelect Depletion and Enrichment Kits provide a fast and efficient method to deplete a specific cell type away from a mixture of cells or remove several unwanted cell populations to enrich for a viable specific cells of interest (negative selection). In both kit types, the cells of interest remain label-free, ensuring cells are not perturbed.

Viable cell-specific populations remain untouched and levitate to the top half of the separation channel while all unwanted populations stay within the LeviCell cartridge. Achieve cell-specific enrichment, dying or dead cell removal—plus removal of debris —in a single 20-minute run.

The LeviSelect portfolio is continually expanding. Inquire to learn about new kits that are available for early access or let us know what cell populations you are interested in isolating.

The LeviSelect Tissue RBC Depletion Kits

Unwanted tissue red blood cells (RBC) removal now can be done lysis-free. The LeviSelect Tissue RBC Depletion Kits for mouse or human can be used to remove contaminating RBCs from primary tissue cell suspensions. Combined with viable cell enrichment, tissue cells can be gently enriched without subjecting cells to unwanted osmolarity changes that can result in premature cell death or transcriptional changes.

Mouse Splenocytes - RBC Depletion

LeviSelect Immune Kits 

LeviSelect Immune Kits support cell-specific of the immune subpopulations required for downstream cell analysis or further subsetting. You can enrich hematopoietic populations quickly without lengthy workflows or potential cell activation.

  • CD3 Enrichment
  • CD4 Enrichment
  • CD8 Enrichment
  • Pan B Enrichment
  • Pan Monocyte Enrichment
  • NK Cell Enrichment

Human PBMCs - hCD4 T Cell Enrichment

LeviSelect CD45 Depletion Kits

For oncology or immuno-oncology research, the LeviSelect CD45 Depletion Kits provide a highly effective method for enriching pure tumor cells enabling characterization for drug discovery and biomarker identification. Human or mouse CD45+ cells are depleted from a mixture of cells in parallel with viable cell enrichment on the LeviCell system. CD45+ cells are retained in the LeviCell cartridge, while CD45- cells of interest remain label-free and are collected for further downstream analysis.

Human Colorectal DTCs - hCD45 depletion

Observe cell-specific enrichment in real time

Experience the enrichment of viable cell-specific cells during the sample processing step like never before. Levitation Technology™ allows you to confidently proceed with your project knowing that unwanted cell depletion has occurred – see it in real time! By using fluorescence labeling, though not required, cells can be visualized as they are depleted away from cells of interest during levitation. In this video, see the Calcein AM (green) stained cells move quickly down to the bottom of the separation channel leaving only the CellTracker Red stained cells levitating in the top channel.

To display RBC removal in realtime, PBMCs were spiked with RBCs that were labeled with Calcein AM and CellTracker Red respectively and run on the LeviCell EOS in parallel. With LeviSelect RBC depletion (Lane2) as compared to no depletion control sample (Lane 3), the red stained RBC band has disappeared from the levitating viable cells in the top fraction. The relative intensity band width, as displayed by LeviMetrics™, is narrower than when the RBCs remain in the sample.

Get specific with LeviSelect

Cell-specific
  • Obtain specific cells of interest
  • Simultaneous depletion of unwanted cells
  • Gentle, efficient, and fast enrichment
Untouched cells
  • No labels
  • Low presses, less stress
  • No cell activation
Simplified workflow
  • Shortened incubation time
  • Remove the need for centrifugation
  • No additional wash steps or lengthy processing time

How it works

LeviSelect Kits combine the power of Levitation Technology with target specific enrichment of cell subtypes. Viable and cell-specific enrichment are performed simultaneously on the LeviCell systems. By combining the natural density and magnetic susceptibility of cells within a paramagnetic compound with the addition of antibodies conjugated to nanospheres of different densities, cells can levitate at different heights. This process allows the separation of specific cells of interest from unwanted cells during a single LeviCell run.

Identifying and enriching cells of interest from mixed cell populations is fundamental to studying disease models and basic cell biology. Levitation Technology provides the gentlest cell enrichment method, preserving target-specific cells of interest unbiasedly.

LeviSelect workflow

Enabling cell-specific enrichment on the LeviCell systems is as easy as viable cell enrichment. Prepare your sample by adding Levitation Agent and LeviSelect reagents. After pre-inserting the cartridge into the instrument, load the sample to enrich for cells of interest. Collect viable cell for downstream applications.

PREPARE SAMPLE

Sample is resuspended.

ADD LEVISELECT REAGENTS & LEVITATION AGENT 

Magnetic nano spheres are added and incubated. Levitation Agent is added.

SAMPLE ENRICHMENTS

Sample is loaded into the cartridge. Targeted viable cell enrichment occurs in the LeviCell system.

SAMPLE COLLECTION

Untouched target specific cells are collected and ready to use for downstream steps and workflows.

End-to-End Levitation-based workflow

Streamline your complete sample processing workflow with Levitation Technology.

 
Access

Access primary cells from tissue samples through cold dissociation

Enrichment

Uncover pure, viable cells free of debris, dead cells and contaminants

Selection

Select cells of interest by modulating levitation through density or particles

Characterization

Characterize levitating samples through Ai-based software tools for actionable insights

Maximize the use of your 20-min run by adding LeviSelect Kits. Talk to a specialist today.

Ordering info

LeviSelect kits

LeviSelect kits can be purchased directly through your local Account Manager. Submit your request here.

Limited supplies of new early access kits are now released. Reserve your kit here.

Depletion Kits

Kit size: 10 rxns/kit

Description:
Depletion of human CD45+ cells from a mixture of cells with simultaneous viable cell enrichment using the LeviCell System.  Human CD45+ cells are retained in the LeviCell cartridge, while the human CD45- cells of interest remain label-free and are collected for further downstream analysis.

  • Includes Human anti-CD45 Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Kit size: 10 rxns / kit

Description:
Depletion of mouse CD45+ cells from a mixture of cells with simultaneous viable cell enrichment using the LeviCell System.  Mouse CD45+ cells are retained in the LeviCell cartridge, while the mouse CD45- cells of interest remain label-free and are collected for further downstream analysis.

  • Includes Mouse anti-CD45 Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Kit size: 10 rxns / kit

Description:
The LeviSelect Mouse Tissue RBC Depletion Kit has been designed to deplete up to 5 million mature erythrocyte and erythroid precursor cells per reaction after a mechanical or enzymatic tissue dissociation while simultaneously enriching for live cells.

  • Includes Mouse RBC Ab Cocktail, SAV Nanospheres, buffer and protocol
  • Average 20 min run for removal of RBCs and enrichment of viable cells away from debris and dead cells

Documents:
Kit size: 10 rxns / kit

Description:
The LeviSelect Human Tissue RBC Depletion Kit has been designed to deplete up to 5 million mature erythrocyte and erythroid precursor cells per reaction after a mechanical or enzymatic tissue dissociation while simultaneously enriching for live cells.

  • Includes Human RBC Ab Cocktail, SAV Nanospheres, buffer and protocol
  • Average 20 min run for removal of RBCs and enrichment of viable cells away from debris and dead cells

Documents:
  • Product Data Sheet (Coming Soon)

Enrichment Kits

Kit size: 10 rxns / kit

Description:
Enrich label-free human CD3 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human CD3 T cells can be used for various downstream applications.  Non-CD3 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Description:
Enrich label-free human CD4 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human CD4 T cells can be used for various downstream applications.  Non-CD4 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Kit size: 10 rxns / kit

Description:
Enrich label-free human CD8 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human CD8 T cells can be used for various downstream applications.  Non-CD8 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Kit size: 10 rxns / kit

Description:
Enrich label-free human Pan B cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human Pan B Cells can be used for various downstream applications.  Non-B cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents
Kit size: 10 rxns / kit

Description:
Enrich label-free human pan monocytes from peripheral blood mononuclear cell (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human pan monocytes can be used for various downstream applications. Non-monocytes are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing step required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Kit size: 10 rxns / kit

Description:
Enrich label-free mouse Pan B cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse Pan B Cells can be used for various downstream applications.  Non-B cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Kit size: 10 rxns / kit

Description:
Enrich label-free mouse CD3 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse CD3 T cells can be used for various downstream applications.  Non-CD3 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Kit size: 10 rxns / kit

Description:
Enrich label-free mouse CD4 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse CD4 T cells can be used for various downstream applications.  Non-CD4 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Kit size: 10 rxns / kit

Description:
Enrich label-free mouse CD8 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse CD8 T cells can be used for various downstream applications.  Non-CD8 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing steps required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Kit size: 10 rxns / kit

Description:
Enrich label-free mouse monocytes from a peripheral blood mononuclear (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse monocytes can be used for various downstream applications. Non-monocytes are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing step required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents:
Kit size: 10 rxns / kit

Description:
Enrich label-free mouse NK cells from a peripheral blood mononuclear cell (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse NK cells can be used for various downstream applications. Non-NK cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

  • Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
  • No centrifugation or washing step required
  • Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Documents: