Levitate untouched viable cells of interest
The LeviSelect™ Kits that are run on the LeviCell® platform enrich for specific cell types of interest and maintain them as untouched, while simultaneously depleting unwanted cells. As with every LeviCell run, removal of debris, dead or dying cells happens concurrently. Bridge complex sample types and successful experiments with Levitation Technology and LeviSelect Kits.
- Built to scale
Targeted depletion and enrichment in one automated step
On the LeviCell 96, LeviSelect assays multiplex with viable cell enrichment. Antibody-mediated negative selection removes unwanted cells while dead cells and debris levitate away, and your cells of interest stay untouched. With kits for immune, tissue and brain samples, one automated run replaces several manual cleanup steps.
- Fewer steps, fewer hand-offs: depletion, enrichment and cleanup together
- Untouched cells with native expression states preserved
- Standard SBS format for 96 samples per run on robotics
LeviSelect Kits
LeviSelect Depletion and Enrichment Kits provide a fast and efficient method to deplete a specific cell type away from a mixture of cells or remove several unwanted cell populations. Enrich for untouched viable specific cells of interest (negative selection) while unwanted cells remain in the cartridge. In both kit types, the cells of interest remain label-free, ensuring cells are not perturbed.
The LeviSelect portfolio is continually expanding. Let us know what cell populations you are interested in isolating.
How it works
LeviSelect Kits combine the power of Levitation Technology with target specific enrichment of cell subtypes. Viable and cell-specific enrichment are performed simultaneously on the LeviCell systems. By combining the natural density and magnetic susceptibility of cells within a paramagnetic compound with the addition of antibodies conjugated to nanospheres of different densities, cells can levitate at different heights. This process allows the separation of specific cells of interest from unwanted cells during a single LeviCell run.
Identifying and enriching cells of interest from mixed cell populations is fundamental to studying disease models and basic cell biology. Levitation Technology provides the gentlest cell enrichment method, preserving target-specific cells of interest unbiasedly.
LeviSelect workflow
Enabling cell-specific enrichment on the LeviCell systems is as easy as viable cell enrichment. Prepare your sample by adding Levitation Agent and LeviSelect reagents. After pre-inserting the cartridge into the instrument, load the sample to enrich for cells of interest. Collect viable cells for downstream applications.
Prepare sample
Sample is resuspended.
Add LeviSelect reagents & Levitation agent
Magnetic nanospheres are added and incubated. Levitation Agent is added.
Sample enrichments
Sample is loaded into the cartridge. Targeted viable cell enrichment occurs in the LeviCell system.
Sample collection
Untouched target specific cells are collected and ready to use for downstream steps and workflows.
Get specific with LeviSelect
Cell-specific
- Obtain specific cells of interest
- Simultaneous depletion of unwanted cells
- Gentle, efficient, and fast enrichment
Untouched cells
- No labels
- Low pressure, less stress
- No cell activation
Simplified workflow
- Shortened incubation time
- Remove the need for centrifugation
- No additional wash steps or lengthy processing time
Complete Mouse Myelin Depletion Kit
The LeviSelect Complete Mouse Myelin Depletion Kit selectively depletes myelin from dissociated mouse brain tissue as well as residual RBCs in a gentle, streamlined workflow. Achieve robust viable cell yields without unwanted contaminants in one single run.
LeviSelect Immune Cell Kits
LeviSelect Immune Cell Kits support cell-specific enrichment of the immune subpopulations required for downstream cell analysis or further subsetting. Enrich hematopoietic populations quickly without lengthy workflows or potential cell activation.
LeviSelect Tissue RBC Depletion Kits
The LeviSelect Tissue RBC Depletion Kits selectively deplete RBCs from mouse or human tissue, avoiding lysis while simultaneously enriching viable cells. Achieve simultaneous depletion of RBCs and enrich for viable cells in one single run.
Ordering info
Are you ready to upgrade your sample preparation and increase data reliability?
LeviSelect kits
LeviSelect kits can be purchased directly through your local Account Manager, or submit your request here.
Depletion Kits
LeviSelect Complete Mouse Myelin Depletion Kit, PN 1004120
Kit size: 10 rxns/kit
Description:
Reagents for mouse brain tissue dissociation and myelin/RBC depletion from <25 mg of starting tissue input. For use with LeviCell systems for complete cell enrichment.
Documents:
LeviSelect Human CD45+ Depletion Kit, PN 1004001
Kit size: 10 rxns/kit
Description:
Depletion of human CD45+ cells from a mixture of cells with simultaneous viable cell enrichment using the LeviCell System. Human CD45+ cells are retained in the LeviCell cartridge, while the human CD45- cells of interest remain label-free and are collected for further downstream analysis.
- Includes Human anti-CD45 Nanospheres, Buffer, and Protocol
- No centrifugation or washing steps required
- Average 20 min run for targeted viable cell enrichment and removal of dead and dying cells and debris
Documents:
LeviSelect Mouse CD45+ Depletion Kit, PN 1004003
Kit size: 10 rxns / kit
Description:
Depletion of mouse CD45+ cells from a mixture of cells with simultaneous viable cell enrichment using the LeviCell System. Mouse CD45+ cells are retained in the LeviCell cartridge, while the mouse CD45- cells of interest remain label-free and are collected for further downstream analysis.
- Includes Mouse anti-CD45 Nanospheres, Buffer, and Protocol
- No centrifugation or washing steps required
- Average 20 min run for targeted viable cell enrichment and removal of dead and dying cells and debris
Documents:
LeviSelect Mouse Tissue RBC Depletion Kit, PN 1004110
Kit size: 10 rxns / kit
Description: The LeviSelect Mouse Tissue RBC Depletion Kit has been designed to deplete up to 5 million mature erythrocytes and erythroid precursor cells per reaction after a mechanical or enzymatic tissue dissociation while simultaneously enriching for live cells.
- Includes Mouse RBC Ab Cocktail, SAV Nanospheres, buffer and protocol
- Average 20 min run for removal of RBCs and enrichment of viable cells away from debris and dead cells
Documents:
LeviSelect Human Tissue RBC Depletion Kit, PN 1004112
Kit size: 10 rxns / kit
Description: The LeviSelect Human Tissue RBC Depletion Kit has been designed to deplete up to 5 million mature erythrocytes and erythroid precursor cells per reaction after a mechanical or enzymatic tissue dissociation while simultaneously enriching for live cells.
- Includes Human RBC Ab Cocktail, SAV Nanospheres, buffer and protocol
- Average 20 min run for removal of RBCs and enrichment of viable cells away from debris and dead cells
Documents:
Enrichment Kits
LeviSelect Human CD3 T Cell Enrichment Kit, PN 1004005
Kit size: 10 rxns / kit
Description: Enrich label-free human CD3 T cells from a peripheral blood mononuclear cell (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human CD3 T cells can be used for various downstream applications. Non-CD3 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.
- Includes Biotinylated-Antibody Cocktail, Streptavidin Nanospheres, Buffer, and Protocol
- No centrifugation or washing steps required
- Average 20 min run for targeted viable cell enrichment and removal of dead and dying cells and debris
Documents:
LeviSelect Human CD4 T Cell Enrichment Kit, PN 1004007
Kit size: 10 rxns / kit
Description: Enrich label-free human CD4 T cells from a peripheral blood mononuclear cell (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human CD4 T cells can be used for various downstream applications. Non-CD4 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.
- Includes Biotinylated-Antibody Cocktail, Streptavidin Nanospheres, Buffer, and Protocol
- No centrifugation or washing steps required
- Average 20 min run for targeted viable cell enrichment and removal of dead and dying cells and debris
Documents:
LeviSelect Human CD8 T Cell Enrichment Kit, PN 1004009
Kit size: 10 rxns / kit
Description: Enrich label-free human CD8 T cells from a peripheral blood mononuclear cell (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human CD8 T cells can be used for various downstream applications. Non-CD8 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.
- Includes Biotinylated-Antibody Cocktail, Streptavidin Nanospheres, Buffer, and Protocol
- No centrifugation or washing steps required
- Average 20 min run for targeted viable cell enrichment and removal of dead and dying cells and debris
Documents:
LeviSelect Human Pan B Cell Enrichment Kit, PN 1004011
Kit size: 10 rxns / kit
Description: Enrich label-free human Pan B cells from a peripheral blood mononuclear cell (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human Pan B Cells can be used for various downstream applications. Non-B cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.
- Includes Biotinylated-Antibody Cocktail, Streptavidin Nanospheres, Buffer, and Protocol
- No centrifugation or washing steps required
- Average 20 min run for targeted viable cell enrichment and removal of dead and dying cells and debris
Documents:
LeviSelect Mouse Pan B Cell Enrichment Kit A, PN 1004019
Kit size: 10 rxns / kit
Description: Enrich label-free mouse Pan B cells from a peripheral blood mononuclear cell (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse Pan B Cells can be used for various downstream applications. Non-B cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.
- Includes Biotinylated-Antibody Cocktail, Streptavidin Nanospheres, Buffer, and Protocol
- No centrifugation or washing steps required
- Average 20 min run for targeted viable cell enrichment and removal of dead and dying cells and debris
Documents: