LeviSelect
Viable cell-specific enrichment
Levitate untouched viable cells of interest
Our LeviCell® 1.0 system has reached a new pinnacle in cell isolation and enrichment by adding our newest product line— the LeviSelect (TM) Enrichment and Depletion Kits.
The LeviSelect™ Kits build on the Levitation Technology™ found in the LeviCell platform by enriching for specific cell types of interest and maintain them as untouched, while simultaneously depleting unwanted cells. As with every LeviCell run, removal of debris, dead or dying cells happens concurrently.
Bridge complex sample types and successful experiments with Levitation Technology and LeviSelect Kits.
LeviSelect Kits
LeviSelect Depletion and Enrichment Kits provide a fast and efficient method to deplete a specific cell type away from a mixture of cells or remove several unwanted cell populations to enrich for a viable specific cells of interest (negative selection). In both kit types, the cells of interest remain label-free, ensuring cells are not perturbed.
Obtain gentle enrichment of viable cell-specific populations, which remain untouched while all unwanted populations stay within the LeviCell cartridge. Achieve cell-specific enrichment with dying or dead cell removal—plus removal of debris —in a single 20-minute run.
LeviSelect Immune Kits support cell-specific of the immune subpopulations required for downstream cell analysis or further subsetting. You can enrich hematopoietic populations quickly without lengthy workflows or potential cell activation.
- CD3 Enrichment
- CD4 Enrichment
- CD8 Enrichment
- Pan B Enrichment
- Pan Monocyte Enrichment
- NK Cell Enrichment
For oncology or immuno-oncology research, the LeviSelect CD45 Depletion Kits provide a highly effective method for enriching pure tumor cells enabling characterization for drug discovery and biomarker identification. Human or mouse CD45+ cells are depleted from a mixture of cells in parallel with viable cell enrichment on the LeviCell system. CD45+ cells are retained in the LeviCell cartridge, while CD45- cells of interest remain label-free and are collected for further downstream analysis.
Get Specific With LeviSelect
Cell-specific
- Obtain specific cells of interest
- Simultaneous depletion of unwanted cells
- Gentle, efficient, and fast enrichment
Untouched cells
- No labels
- Low presses, less stress
- No cell activation
Simplified workflow
- Shortened incubation time
- Remove the need for centrifugation
- No additional wash steps or lengthy processing time
How it works
LeviSelect Kits combine the power of Levitation Technology with target specific enrichment of cell subtypes. Viable and cell-specific enrichment are performed simultaneously on the LeviCell systems. By combining the natural density and magnetic susceptibility of cells within a paramagnetic compound with the addition of antibodies conjugated to nanospheres of different densities, cells can levitate at different heights. This process allows the separation of specific cells of interest from unwanted cells during a single LeviCell run.
Identifying and enriching cells of interest from mixed cell populations is fundamental to studying disease models and basic cell biology. Levitation Technology provides the gentlest cell enrichment method, preserving target-specific cells of interest unbiasedly.
LeviSelect workflow
Enabling cell-specific enrichment on the LeviCell systems is as easy as viable cell enrichment. Prepare your sample by adding Levitation Agent and LeviSelect reagents. After pre-inserting the cartridge into the instrument, load the sample to enrich for cells of interest. Collect viable cell for downstream applications.
PREPARE SAMPLE
Sample is resuspended.
ADD LEVISELECT REAGENTS & LEVITATION AGENT
Magnetic nano spheres are added and incubated. Levitation Agent is added.
SAMPLE ENRICHMENTS
Sample is loaded into the cartridge. Targeted viable cell enrichment occurs in the LeviCell system.
SAMPLE COLLECTION
Untouched target specific cells are collected and ready to use for downstream steps and workflows.
Figure 1. Three tissues (perfused brain, liver, lung) were harvested from 24 hour old mouse and dissociated using LeviPrep Mouse Tissue Dissociation Kit. Cold dissociation protocol ensures viability and cell health is maintained. Cells were subsequently run on the LeviCell system to further improve viability.
End-to-End Levitation-based workflow
Streamline your complete sample processing workflow with Levitation Technology.
Access
Access primary cells from tissue samples through cold dissociation
Enrichment
Uncover pure, viable cells free of debris, dead cells and contaminants
Selection
Select cells of interest by modulating levitation through density or particles
Characterization
Characterize levitating samples through Ai-based software tools for actionable insights
Ordering info
LeviSelect kits
LeviSelect kits can be purchased directly through your local Account Manager. Submit your request here.
Limited supplies of 6 new early access kits are now released. Reserve your kit here.
- LeviSelect Human Pan Monocyte Enrichment Kit
- LeviSelect Mouse CD3 T Cell Enrichment Kit
- LeviSelect Mouse CD4 T Cell Enrichment Kit
- LeviSelect Mouse CD8 T Cell Enrichment Kit
- LeviSelect Mouse Monocyte Enrichment Kit
- LeviSelect Mouse NK Cell Enrichment Kit
Depletion Kits
Kit size: 10 rxns/kit
Description:
Depletion of human CD45+ cells from a mixture of cells with simultaneous viable cell enrichment using the LeviCell System. Human CD45+ cells are retained in the LeviCell cartridge, while the human CD45- cells of interest remain label-free and are collected for further downstream analysis.
- Includes Human anti-CD45 Nanospheres, Buffer, and Protocol
- No centrifugation or washing steps required
- Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris
Documents:
Kit size: 10 rxns / kit
Description:
Depletion of mouse CD45+ cells from a mixture of cells with simultaneous viable cell enrichment using the LeviCell System. Mouse CD45+ cells are retained in the LeviCell cartridge, while the mouse CD45- cells of interest remain label-free and are collected for further downstream analysis.
- Includes Mouse anti-CD45 Nanospheres, Buffer, and Protocol
- No centrifugation or washing steps required
- Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris
Documents:
Enrichment Kits
Kit size: 10 rxns / kit
Description:
Enrich label-free human CD3 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human CD3 T cells can be used for various downstream applications. Non-CD3 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.
- Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
- No centrifugation or washing steps required
- Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris
Documents:
Description:
Enrich label-free human CD4 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human CD4 T cells can be used for various downstream applications. Non-CD4 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.
- Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
- No centrifugation or washing steps required
- Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris
Documents:
Kit size: 10 rxns / kit
Description:
Enrich label-free human CD8 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human CD8 T cells can be used for various downstream applications. Non-CD8 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.
- Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
- No centrifugation or washing steps required
- Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris
Documents:
Kit size: 10 rxns / kit
Description:
Enrich label-free human Pan B cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human Pan B Cells can be used for various downstream applications. Non-B cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.
- Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
- No centrifugation or washing steps required
- Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris
Documents
Kit size: 10 rxns / kit
Description:
Enrich label-free human pan monocytes from peripheral blood mononuclear cell (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human pan monocytes can be used for various downstream applications. Non-monocytes are magnetically labeled and remain immobilized inside the LeviCell cartridge.
- Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
- No centrifugation or washing step required
- Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris
Documents:
- Product Data Sheet (Coming Soon)
- Safety Data Sheets
Kit size: 10 rxns / kit
Description:
Enrich label-free mouse Pan B cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse Pan B Cells can be used for various downstream applications. Non-B cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.
- Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
- No centrifugation or washing steps required
- Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris
Documents:
Kit size: 10 rxns / kit
Description:
Enrich label-free mouse CD3 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse CD3 T cells can be used for various downstream applications. Non-CD3 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.
- Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
- No centrifugation or washing steps required
- Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris
Documents:
- Product Data Sheet (Coming Soon)
- Safety Data Sheets
Kit size: 10 rxns / kit
Description:
Enrich label-free mouse CD4 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse CD4 T cells can be used for various downstream applications. Non-CD4 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.
- Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
- No centrifugation or washing steps required
- Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris
Documents:
- Product Data Sheet (Coming Soon)
- Safety Data Sheets
Kit size: 10 rxns / kit
Description:
Enrich label-free mouse CD8 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse CD8 T cells can be used for various downstream applications. Non-CD8 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.
- Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
- No centrifugation or washing steps required
- Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris
Documents:
- Product Data Sheet (Coming Soon)
- Safety Data Sheets
Kit size: 10 rxns / kit
Description:
Enrich label-free mouse monocytes from a peripheral blood mononuclear (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse monocytes can be used for various downstream applications. Non-monocytes are magnetically labeled and remain immobilized inside the LeviCell cartridge.
- Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
- No centrifugation or washing step required
- Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris
Documents:
- Product Data Sheet (Coming Soon)
- Safety Data Sheets
Kit size: 10 rxns / kit
Description:
Enrich label-free mouse NK cells from a peripheral blood mononuclear cell (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable mouse NK cells can be used for various downstream applications. Non-NK cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.
- Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
- No centrifugation or washing step required
- Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris
Documents:
- Product Data Sheet (Coming Soon)
- Safety Data Sheets