Uncover the role of immune cells in disease biology
The understanding of the immune system is directly tied to current and future therapeutic approaches regarding human health. The inherent complexity of the immune system, and the critical role it plays in disease pathology, presents scientists with both technical and analytical hurdles that require optimized sample preparation.
Levitation Technology™ on the LeviCell® platform provides a streamlined workflow to obtain untouched immune cells for immunology, oncology, immuno-oncology and drug discovery applications.
- Built to scale
Ready for automated, 96-well workflows
Immune profiling studies are growing to hundreds of donors and conditions. The LeviCell 96 runs label-free enrichment alongside targeted depletion for up to 96 samples per run on standard SBS-compatible robotics, giving you higher-quality inputs, more samples per day and a lower cost per sample.
- Combined in one run: depletion, viable cell enrichment and debris removal
- Consistent well to well, without manual variability
- High-fidelity data for large-scale studies
Levitate untouched viable cells of interest with LeviSelect™ Depletion Kits
LeviSelect™ Depletion Kits provide a fast and efficient method to deplete a specific cell type away from a mixture of cells or remove several unwanted cell populations. Enrich for untouched viable specific cells of interest (negative selection) while unwanted cells remain in the LeviCell cartridge. The cells of interest remain label-free, ensuring cells are not perturbed.
Observe cell-specific enrichment in real time
Experience the enrichment of viable cell-specific cells during the sample processing step like never before. Levitation Technology allows you to confidently proceed with your project knowing that unwanted cell depletion has occurred – see it in real time!
By using fluorescence labeling, though not required, cells can be visualized as they are depleted away from cells of interest during levitation. In this video, see the Calcein AM (green) stained cells move quickly down to the bottom of the separation channel leaving only the CellTracker™ Red stained cells levitating in the top channel.
Flow intensity histogram plots of LeviSelect Human CD45 depletion
LeviSelect CD45 Depletion Kits
For oncology and immuno-oncology research, the LeviSelect CD45 Depletion Kits enrich pure tumor cells for drug discovery and biomarker identification. Human or mouse CD45+ cells are depleted from a mixture of cells in parallel with viable cell enrichment. CD45+ cells are retained, while the CD45- cells of interest stay label-free and are collected for downstream analysis.
Human colorectal DTCs, hCD45 depletion
Ordering info
Are you ready to upgrade your sample preparation and increase data reliability?
LeviSelect kits
LeviSelect kits can be purchased directly through your local Account Manager.
Depletion Kits
LeviSelect CD45 Depletion Kits are available in a 10-reaction cartridge format for the LeviCell 1.0, 1.0 Access and EOS, and in a 96-reaction format for the LeviCell 12 and LeviCell 96 SALT™ arrays.
LeviSelect Human CD45+ Depletion Kit, PN 1004001
Kit size: 10 rxns/kit
Description:
Depletion of human CD45+ cells from a mixture of cells with simultaneous viable cell enrichment using the LeviCell System. Human CD45+ cells are retained in the LeviCell cartridge, while the human CD45- cells of interest remain label-free and are collected for further downstream analysis.
- Includes Human anti-CD45 Nanospheres, Buffer, and Protocol
- No centrifugation or washing steps required
- Average 20 min run for targeted viable cell enrichment and removal of dead and dying cells and debris
Documents:
LeviSelect Mouse CD45+ Depletion Kit, PN 1004003
Kit size: 10 rxns / kit
Description:
Depletion of mouse CD45+ cells from a mixture of cells with simultaneous viable cell enrichment using the LeviCell System. Mouse CD45+ cells are retained in the LeviCell cartridge, while the mouse CD45- cells of interest remain label-free and are collected for further downstream analysis.
- Includes Mouse anti-CD45 Nanospheres, Buffer, and Protocol
- No centrifugation or washing steps required
- Average 20 min run for targeted viable cell enrichment and removal of dead and dying cells and debris
Documents:
LeviSelect Human CD45+ Depletion Kit, 96 rxn (LeviCell 12 / 96 format), PN 1004002
Kit size: 96 rxns / kit
Description: The 96-reaction format of the LeviSelect Human CD45+ Depletion Kit for the SALT™ array: human CD45+ cells are depleted while viable CD45- cells of interest are enriched, on up to 96 samples per run.
LeviSelect Mouse CD45+ Depletion Kit, 96 rxn (LeviCell 12 / 96 format), PN 1004004
Kit size: 96 rxns / kit
Description: The 96-reaction format of the LeviSelect Mouse CD45+ Depletion Kit for the SALT™ array: mouse CD45+ cells are depleted while viable CD45- cells of interest are enriched, on up to 96 samples per run.