Courtney Ward
Core Facility Manager
Queen's University Belfast
The LeviCell EOS system has been a key addition to our scRNA-seq workflow. It allows us to visually QC samples in detail ahead of loading on to the 10X Genomics Chromium X system. The data obtained indicates sample suitability for further analysis, preventing us from proceeding with sub-optimal samples and thus saving both our unit and our users significant time and money.
Nidal Karagic, PhD
Postdoctoral Researcher
Institute of Biotechnology, University of Helsinki
Thanks to the LeviCell system, our single cell experiments have seen a significant increase in viable cell recovery. Fragile cells are handled with care resulting in 2-3 times higher yields!
Greg Golden, PhD
Postdoctoral Researcher
University of Pennsylvania
The LeviCell EOS system allows our lab to process several low-viability samples simultaneously into high-viability samples, which is absolutely critical for single cell sequencing analyses. The LeviCell EOS system will increase our throughput and quality for rare and difficult samples.
Michelle Galeas-Peña, MD, PhD
Research Assistant Professor
Tulane University School of Medicine
The LeviCell 1.0 system is easy and intuitive to use, enabling us to enrich for precious viable cells from cell suspensions with viabilities below 40%, dissociated from frozen biobanked tissues for scRNA-seq.
Stefan Günther, PhD
Head of Next Gen Sequencing Facility
Max Planck Institute
We successfully integrated LevitasBio’s Levitation Technology into our workflows for a variety of single cell approaches. Experiments with very sensitive cell types, or samples that are not suitable for cell sorting, benefit from this gentle, fast method to exclude dead cells and debris.
- New study: Discovery Life Sciences × Parse Biosciences
10× the usable data. 1/10th the cost. One 40-minute run.
Discovery Life Sciences levitated 16 cryopreserved dissociated tumor samples, then sequenced them with Parse Evercode WT. Live cells floated into a band while dead cells and debris sank, with no labels, beads or centrifugation.
1 → 10
of 16 samples cleared the 70% viability gate
89%
of dead cells removed in a typical sample
6×
fewer doublets in Parse sequencing
- Why LevitasBio
Better data, more samples, lower cost and hands off the pipette
Sample prep is where data quality is won or lost, and it is still the most manual step in the lab. Levitation Technology™ replaces centrifuges, filters, beads and labels with a gentle magnetic density field, so the step that once needed hands now runs on a robot.
Data quality
Over 95% viability, debris and dead cells removed, and no labels, pressure or shear that could alter expression states.
Throughput
96 samples in parallel on one array: 5 minutes hands-on, 40 minutes of levitation, 45 minutes from loading to enriched cells.
Cost per sample
About $10 per sample instead of more than $100, with fewer failed runs and repeats downstream.
Automation
A standard SBS footprint, scripted deck protocols, three hands-on steps and walkaway runs.
- One workflow
Every sample-prep step, on the robot deck
The LeviCell® 96 System brings the steps that usually happen on separate benches into one label-free, centrifuge-free workflow on standard SBS-compatible liquid handling.
- Cells: primary cells, PBMCs, dissociated tumors, adherent and suspension lines
- Nuclei: isolated, washed and eluted in sequential wells
- Organoids and spheroids: whole or dissociated
- Particles: from bacteria to large clusters, sorted by density
Enrich
Separate viable cells from dead cells and debris.
Wash
Buffer exchanges in the well, with no centrifuge spins.
Pellet
Levitation gathers viable cells into a tight pellet above dead cells and debris, with no centrifuge.
Image
Optional imaging shows every sample as it levitates.
Analyze
Quantify viability, debris and fractions per well.
Collect
Pick the fraction of interest and leave the waste behind.
Pool
Combine wells or replicates to fit your study design.
Concentrate
Elute in small volumes, ready for loading.
- Data at scale
High-fidelity data for the large models everyone is chasing
Virtual cell models, perturbation atlases and foundation-scale single-cell and organoid datasets need millions of cells. Every one of them has to be prepared the same way, on the first try. Manual prep can't deliver that kind of consistency. Automated levitation can.
96
samples processed in parallel, well after well
>95%
viability going into sequencing, cytometry or screening
3
hands-on steps, from operator to robot
$10
per sample, so million-cell studies fit the budget
Reproducible by design
Identical, scripted processing in every well removes operator-to-operator and day-to-day variability, a major source of batch effects.
Clean, unbiased inputs
Label-free separation preserves native biology and removes dead cells and debris, which add noise to downstream data.
Sample-level metadata
Imaging during levitation gives each sample a visual QC record and quantitative metrics to store alongside its downstream data.
- Built for automation
Drops into the automation you already run
The Selective Arrayed Levitation Technology (SALT™) array uses the SLAS/SBS 96-well footprint, so it fits a standard deck position next to your tips, plates and reagents.
- No centrifuge, filters, beads or microfluidics: fewer devices to integrate and fewer moving parts
- Scripted protocols: enrichment, wash and nuclei workflows run on the liquid handler
- 15,000 to 500,000 cells per well: from precious biopsies to bulk screening
- Same chemistry from 12 to 96: develop methods on the LeviCell 12, then scale up to the LeviCell 96 without re-optimizing
- Applications
Applied across your research needs
Viable Cell Enrichment
Rescue precious samples even at low viabilities and low quantities while simultaneously removing debris and contaminants.
Single-Cell Genomics
Obtain high quality cells and yield more cells, more sequencing data and more significant results.
Cancer Profiling
Unmask the true biology behind cancer by characterizing the cellular and molecular basis of tumors.
3D Cultures
Standardize organoid and spheroid workflows in 96-well format for reproducible screening, toxicity and disease modeling at scale.