Realize a lysis-free workflow
Unwanted red blood cells (RBC) can now be effectively removed from tissue in a lysis-free manner. The LeviSelect™ Tissue RBC Depletion Kits for mouse or human can be used to remove contaminating RBCs from primary tissue-derived single-cell suspensions. Combined with viable cell enrichment on the LeviCell® platform, sample and data quality is greatly improved as compared to methods which utilize lysis.
- Avoid harsh lysis conditions
- Deplete developing and mature RBCs from tissue
- Reduce variability and hands on time
- Improve accuracy of sample quantification
- Increase data quality and resolution
- Built to scale
Ready for automated, 96-well workflows
Lysis-free RBC depletion removes a harsh, variable manual step from tissue workflows. The LeviCell 96 runs label-free enrichment alongside targeted depletion for up to 96 samples per run on standard SBS-compatible robotics, giving you higher-quality inputs, more samples per day and a lower cost per sample.
- Combined in one run: depletion, viable cell enrichment and debris removal
- Consistent well to well, without manual variability
- High-fidelity data for large-scale studies
Levitate untouched viable cells of interest with LeviSelect
LeviSelect Depletion and Enrichment Kits provide a fast and efficient method to deplete a specific cell type away from a mixture of cells or remove several unwanted cell populations. Enrich for untouched viable specific cells of interest (negative selection) while unwanted cells remain in the LeviCell cartridge. In both kit types, the cells of interest remain label-free, ensuring cells are not perturbed.
RBC removal in real-time
To display RBC removal in real-time, PBMCs were spiked with RBCs that were labeled with Calcein AM and CellTracker Red respectively and run on the LeviCell EOS in parallel. With LeviSelect RBC depletion (Lane 2) as compared to no depletion control sample (Lane 3), the red stained RBC band has disappeared from the levitating viable cells in the top fraction. The relative intensity band width, as displayed by LeviMetrics™, is narrower than when the RBCs remain in the sample.
Remove RBCs and obtain better sample quality
Every workflow step can introduce variation, cell loss, apoptotic pathways, or change gene expression profiles. Using Levitation Technology™ and the LeviCell platform, researchers can now streamline their workflow when processing primary tissue by simultaneously removing RBC contamination from tissue and enriching for viable cells of interest.
Mouse Splenocytes - RBC Depletion
Reliable, agnostic method
Confidently remove RBCs from a variety of tissue types and rescue low-quality or limited quantity samples. Tested with mouse lung, spleen (as shown in the figure), pancreas, brain, and liver – maximize sample and assay potential using Levitation Technology.
- Exceptional RBC removal from varying sample input ranges
- Simultaneous removal of debris and dead/dying cells
- Improved viability and overall sample quality
Increase sequencing resolution
RBC lysis results in high levels of ambient RBC-associated genes such as Hba-a1. As a result, background noise can be seen across all cell populations which require filtering (as seen in the figure with mouse splenocytes). The remaining signal, after filtering, displays incomplete lysis of precursor or mature RBCs.
With LeviSelect Tissue RBC Depletion, there is complete removal of any ambient RNA signal providing high quality sequencing resolution of only the cells of interest.
Reduce wasted reads and bring true biology into focus
With LeviSelect’s Tissue RBC Depletion Kit, both precursor and mature RBCs are removed simultaneously with viable cell enrichment. As seen with scRNA-seq, a significant portion of reads are wasted using RBC lysis.
Maximize resources by sequencing only the cells of interest for higher resolution.
- Remove lysis-resistant erythroblasts and mature RBCs
- Maintain and recover target cell populations
- Sequence highly viable cells
- Minimize process-related artifacts
Ordering info
Are you ready to upgrade your sample preparation and increase data reliability?
LeviSelect kits
LeviSelect kits can be purchased directly through your local Account Manager, or submit your request here.
Depletion Kits
LeviSelect Tissue RBC Depletion Kits are available in a 10-reaction cartridge format for the LeviCell 1.0, 1.0 Access and EOS, and in a 96-reaction format for the LeviCell 12 and LeviCell 96 SALT™ arrays.
LeviSelect Mouse Tissue RBC Depletion Kit, PN 1004110
Kit size: 10 rxns / kit
Description: The LeviSelect Mouse Tissue RBC Depletion Kit has been designed to deplete up to 5 million mature erythrocytes and erythroid precursor cells per reaction after a mechanical or enzymatic tissue dissociation while simultaneously enriching for live cells.
- Includes Mouse RBC Ab Cocktail, SAV Nanospheres, buffer and protocol
- Average 20 min run for removal of RBCs and enrichment of viable cells away from debris and dead cells
Documents:
LeviSelect Human Tissue RBC Depletion Kit, PN 1004112
Kit size: 10 rxns / kit
Description: The LeviSelect Human Tissue RBC Depletion Kit has been designed to deplete up to 5 million mature erythrocytes and erythroid precursor cells per reaction after a mechanical or enzymatic tissue dissociation while simultaneously enriching for live cells.
- Includes Human RBC Ab Cocktail, SAV Nanospheres, buffer and protocol
- Average 20 min run for removal of RBCs and enrichment of viable cells away from debris and dead cells
Documents:
LeviSelect Mouse Tissue RBC Depletion Kit, 96 rxn (LeviCell 12 / 96 format), PN 1004111
Kit size: 96 rxns / kit
Description: The 96-reaction format of the LeviSelect Mouse Tissue RBC Depletion Kit for the SALT™ array, for lysis-free RBC depletion and viable cell enrichment on up to 96 samples per run.
LeviSelect Human Tissue RBC Depletion Kit, 96 rxn (LeviCell 12 / 96 format), PN 1004113
Kit size: 96 rxns / kit
Description: The 96-reaction format of the LeviSelect Human Tissue RBC Depletion Kit for the SALT™ array, for lysis-free RBC depletion and viable cell enrichment on up to 96 samples per run.