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LeviPrep Nuclei Kit
- Proprietary extraction method
- Gain access to limited & archived samples
- Enrich for high-quality nuclei
A radical new approach for nuclei enrichment
Leveraging Levitation Technology™, achieve consistent and gentle separation of unlabeled nuclei from sample debris on the LeviCell® systems. Avoid losing any sample data points by using only highly pure nuclei.
Remove contaminants that can affect downstream single-nuclei sequencing results for both gene expression or epigenetic analysis – forging research discovery forward in multi-omic analysis.
Simplified workflow
With a few simple steps, the LeviPrep™ Nuclei Kit enables a fast, easy, and reliable enrichment alternative for nuclei from a variety of starting materials. The LeviPrep Nuclei Kit efficiently extracts nuclei from tissue while protecting nucleic acid integrity prior to the automated enrichment process on the LeviCell® systems.
Leverage the LeviCell System for improved workflow and data
- Gentle, easy, and reliable method for obtaining high-quality nuclei
- Unique technology removes excessive debris and nuclear aggregates
- Automated enrichment processes free up time and reduces variability
Results you can see: visibly uniform and intact nuclei
Suboptimal extraction protocols with no enrichment often yields problematic nuclear clumping along with significant amounts of debris and background noise. Enriched nuclei using the LeviPrep Nuclei Kit generates visibly uniform and intact nuclei with a significant reduction of clumping and debris contamination.
Buffer/Wash Only
LeviPrep Nuclei Kit
Improved sequencing results
When examining single nuclei sequencing results and comparing to a competitor kit, we see that the LeviPrep kit results in a more consistent number of nuclei detected relative to the number of nuclei actually targeted, which is critical for downstream analysis. Counting nuclei can be confounded due to excess debris in the sample post extraction. Cleaner, more homogenous nuclei samples, yield better counting thereby can lead to higher more consistent reads per cell. Variations in targeted nuclei can cause a reduction in the mean reads per nuclei. Lastly, with high quality samples, there is a lower number of nuclei removed from the data set due to high mitochondrial reads – making the best use of sequencing dollars spent on the experiment.
Resources
Discover the advantages that researchers have
experienced using Levitation Technology by reading our research snapshot and application note.
Ordering info
Description:
Pre-optimized kit for extraction and enrichment of nuclei from fresh or frozen tissue and cells. This kit allows for gentle extraction and preservation of nuclei and clean up of debris ideal for down stream assays like single nuclei sequencing.
- Includes all reagents and plastic consumables for 16 extractions
- Includes reagents for nuclei enrichment for use on the LeviCell systems
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