Cancer Profiling

Unmasking the true biology behind cancer

Unmasking the true biology behind cancer

Tumors are composed of diverse cell subpopulations with distinct genotypes, phenotypes, and treatment sensitivities. Tumor heterogeneity is further complicated by the formation of microenvironmental niches, such as necrotic regions, which often promote the selection of more aggressive cancer cell clones. This complexity and associated challenges can hinder researchers and clinicians from fully understanding the molecular basis of tumors and developing effective treatments. Advanced tools and techniques are urgently needed to overcome these obstacles and drive scientific breakthroughs.

High-quality data demands high-quality samples

Recent advancements in analytical techniques for research and drug discovery have significantly deepened our understanding of the cellular and molecular basis of tumors. These include a variety of cell-based assays such as growth assays, killing assays, and metabolic profiling, as well as the development of xenograft models and the emergence of advanced single-cell sequencing technologies. These assays are directly linked to the quality of the sample input. Using low-quality input material compromises reproducibility and data validity and can trigger a cascade of adverse events impacting the assay or the entire project.

Achieve high-quality samples with the best sample prep method

Overcome hurdles in cancer profiling with Levitation Technology

Levitation Technology™ offers an innovative and efficient alternative to traditional cell separation methods, significantly enhancing the accuracy and reliability of cell-based assays. By employing a paramagnetic solution within a magnetic field, the system levitates viable cells, effectively separating them from dead or dying cells without labels. The streamlined process involves three simple steps and is completed in just 20 minutes, ensuring high cell viability and yield, which are crucial for precise tumor characterization assays.

Unlike conventional methods which use harsh treatments that might result in the loss of cells of interest, Levitation Technology provides a gentle, label-free approach to cell enrichment, minimizing cell stress and preserving cell integrity.

The simple, three-step process takes just 20 minutes

The result? High-quality, viable cells and data that you can trust!

Sample preparation for consistent and robust oncology workflows

Levitation Technology ensures high-quality cell preparation, providing consistent and reliable results across a wide range of oncology workflows, including:

Transform cancer research workflows and results

Levitation Technology offers a revolutionary solution for cell separation, addressing the limitations of traditional techniques. There are numerous key advantages of using Levitation Technology in cancer profiling workflows, including:

High viability and yield

Levitation Technology ensures high cell viability and yield by gently separating viable cells from dead or dying cells without labels, preserving cell integrity. In collaboration with Discovery Life Sciences, 45 complex human samples spanning 14 cancer types, the LeviCell® system achieved an average viability of 80%, boosting sample viability by 25%.

Improved signal-to-noise ratio

Removing dead cells and debris results in a higher signal-to-noise ratio, which is crucial for precision applications like single-cell RNA sequencing, cell therapy development, and drug dosing assays. With a starting viability of 49%, cryo perserved DTCs from a sarcomatoid carcinoma lung cancer improved to 80% viability after levitation. After targeting 5000 cells, sc RNA Seq data is markedly improved in several defining sequencing QC metrics.

Tumor representation

The LeviCell systems preserve all cell types at representative ratios due to its broad cell size compatibility and focus on maintaining integrity. Through LeviSelect™ negative selection and viability enrichment, cancer researchers can achieve a 6x representation of relevant cancer cells and 99.9% depletion of unwanted contaminants like CD45+ cells.

Enhanced model development

By improving the quality and viability of cells, Levitation Technology supports better engraftment efficiency and disease progression in xenograft models. The LeviCell workflow reduced the cells needed for cell-derived xenograft models from 1-2 million at mixed viability to 100,000 at 95% viability. Moreover, it improved engraftment and disease progression rates from 80% and 50%, respectively, to 100%.

A revolutionary solution for cell separation

Time efficiency

The cell separation process is streamlined into three simple steps that can be completed in just 20 minutes, significantly reducing timelines and increasing lab productivity.

Cost-effectiveness

Higher-quality starting material reduces the need for repeat experiments, extensive data filtering, or corrections, optimizing resource use while aligning with ethical standards by minimizing animal use.

Consistency and reproducibility

Consistent, high-quality cell populations lead to more reproducible results in assays and model development, improving overall research outcomes.

Reliable assay outcomes

High-viability cells reduce variability in assay results, which is particularly important for drug efficacy evaluations and single-cell analyses, ensuring accurate and meaningful data.

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