Hepatocyte Isolation and Purification – A Worldwide Research Priority

The largest solid organ in the body is the liver. Made up primarily (i.e., at least 80%) of hepatocytes of various types, the liver is the site of hepatocellular carcinoma (HCC), the fourth ranked cause of cancer deaths worldwide. A large and growing HCC caseload has led to a vast World-wide research effort to better […]

Improve Sample Quality For RNA Sequencing Through Quality Control Checks

Ideally, the integrity and purity of RNA in a tissue sample should be established prior to nuclei isolation. While intact nuclei can be isolated from many tissues, in some instances the RNA associated with those nuclei will be too degraded and thus not suitable for subsequent analyses. Of particular concern, if RNA obtained from frozen […]