New Partnership: LevitasBio and Cytiva

Flow Cytometry

Analyze more viable cells, reduce dead cell and debris

Start with Viable, Debris-free Cells–Every Time

Flow cytometry is an important and powerful cellular research method; however, sample quality and sensitivity can hinder the flow process, and researchers often face a dilemma between speed vs. cell stress. Higher pressure creates faster results but can also be less accurate and more damaging to fragile and sensitive cells. Not anymore.

The advanced LeviCell® 1.0 system allows flow cytometry users to perform a simple, 3-step, 20 min walkaway viable enrichment run that enables faster processing time and improved sample resolution independent of dead cells and debris, initial sample viability, and initial starting cell numbers.

 

  • Reduce dead cells and debris by 5x
  • Boost viable cell count 3x
  • Start with as few as 8000 cells*

*results may vary based on starting sample

With LeviCell, you can shorten flow analysis and sorting times, improve scRNA-seq performance metrics, and minimize processing-induced stress, unwanted activation, and unnecessary cell loss.

Any size, any type

Untouched & label-free

Simplified workflow

Powered by Revolutionary Levitation Technology

Developed at Stanford and Harvard Universities, LeviCell systems use revolutionary Levitation Technology™, which is based on individual cells’ intrinsic properties of density and buoyancy. Physical signatures of cells vary based on cell type and state, causing them to levitate in predictable ways when in a magnetic field. The LeviCell systems capitalize on this quality to separate cells based on cell properties and varied levitation heights.

Expanding Your Flow Capabilities with Efficient, Effective Viable Cell Enrichment

In the example below, Human Lung DTC samples were processed for viable cell enrichment using the LeviCell system. The viability of the post-Levicell DTC cell suspension increased approximately 3-fold, from 32% to 87%, due to the removal of dead cells, which decreased over 5-fold, from 67% to 12%.

Before LeviCell:
After LeviCell:

Conclusion

The innovative label-free separation technology of the LeviCell systems facilitate complete debris and dead cell removal without affecting the original population representation of gene expression (please see our Population Representation application note for additional data on how the LeviCell maintains the original population representation).

When it comes to single-cell study, purity and viability of cells harvested from the sample preparation stage is a determining factor for generating high quality data in downstream assays such as NGS (Next Generation Sequencing).

The LeviCell systems ability to seamlessly enrich for target cells and produce robust yields of viable cells without preferentially depleting or changing the frequency or expression of cell types gives it the necessary technological characteristics that many scientists have been waiting for to take them to the next level.

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Request a quote or reach out today to see how Levitation Technology can support your workflow