Single cell sequencing is a relatively recent approach that enables scientists to study biological pathways at unprecedented resolution. When preparing single cell suspensions for downstream experiments such as single cell RNA sequencing (scRNA-seq), researchers must optimize steps from cell dissociation through library preparation to get quality results.

In this Technique Talk brought to you by The Scientist, Arpita Kulkarni from Harvard Medical School’s Single Cell Core will share a practical guide on how to design a successful scRNA-seq experiment from the wet bench perspective.

Learning Objectives

• Types of single cell sequencing and different -omics that scientists can study at single cell resolution
• Understanding the basics of scRNA-seq, how it differs from bulk RNA-seq, and when to choose this method over other approaches
• Key sample preparation considerations and best practices for designing and carrying out a scRNA-seq workflow
• Choosing scRNAseq platforms and combining single cell sequencing with other technologies
• Analyzing single cell data

About Dr. Arpita Kulkarni:

Arpita Kulkarni is associate director of the Single Cell Core at Harvard Medical School. As a cell and developmental biologist, Kulkarni uncovers the diverse mechanisms in which normal developmental pathways go awry in disease, elucidating disease etiology at single cell resolution. She leverages cell biology, genomics, medical engineering, and single cell and spatial technologies for translational research applications, including therapeutic development in oncology and neuro-psychiatry.