Dissociated Tumor Cells
The key to unlocking targeted cancer therapies that will improve patient survival lies in the isolation and analysis of live cells in the tumor microenvironment. As primary cells that offer the closest representation of the original cancerous tissue, dissociated tumor cells (DTC) contain tumor, immune, and support cells. Each cell type plays a distinct role in tumorigenesis. Hence, the preservation of each cell type’s population ratio within the microenvironment is vital to the integrity of any downstream application and analysis.
Working with dissociated tumor cell samples is known to be technically challenging, involves rigorous incubation periods, and is associated with low cellular integrity and viability for downstream assay use. Traditionally, the dissociation process is performed using a combination of enzymatic and mechanical separation, which often result in the damage and degradation of cells. Consequently, DTC samples contain a high percentage of debris that interferes with post-processing analysis.
The LeviCellTM removes debris and enriches for live cells while preserving each unique cell type’s population ratio within the original tumor microenvironment. The aggravating issue of contamination is eliminated by having the entire dissociation process occur in the LeviCellTM’s closed, touch-free, self-contained environment. Furthermore, the LeviCellTM’s ability to produce robust, viable cells and abundant yield removes the tedious need for serial subculturing to enrich the tumor cells, thus reducing the overall financial burden and time investment associated with dissociating tumor cells.
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LevitasBio developed a revolutionary levitation technology platform that uses less than 1 psi of pressure to enable a completely touch-free, label-free, enzyme-free, three-step dissociation process that produces high DTC yield without compromising or biasing cell type populations in the original microenvironment.
Sample pipetted into the LeviCell™ cartridge.
Automated Label-Free Sorting
Precision density gradients drive levitation based on cells physical properties.
Selected populations collected into separate ports for further analysis or use.
Viable DTCs are an integral component of therapeutic, diagnostic, and preclinical cancer research. Current methods used for sorting dissociated tumor cells take a certain level of finesse and technical expertise, time, and ample funding to produce sufficient yield and DTC viabilities that enable the analysis of these rare and sensitive cells.
The LeviCellTM’s 3-step, hands- and label-free platform enables the enrichment of DTCs while maintaining their gene expression and original sample representation ratio for accurate downstream analysis. This unique combination of technological features enables scientists and researchers to focus their effort and expertise on advancing their life-saving scientific knowledge and discoveries.