A frozen vial of dissociated tumor cells (DTCs) was thawed in a water bath at 37ᵒC. With a small amount of ice remaining in the vial, the thawed cells were transferred into 10 mL of warmed media + 10% FBS dropwise. Cells were centrifuged at 500 rcf for 5 minutes. The supernatant was removed, and the pellet was resuspended in 10 mL of warm media. Cell count and viability were determined via Trypan blue staining at a 1:1 dilution.
The LeviCellTM Yield Difference
Unmatched Debris Removal
A list of tissue types tested on the LeviCellTM:
The LeviCellTM’s innovative levitation technology platform uses less than 1 psi of pressure to enable a completely touch-free, label-free, three-step gentle sorting process that takes only 20 minutes to complete. The system is truly a simple, fast, and reliable method for working with the complicated and traditionally difficult to process dissociated tumor samples for live-cell enrichment and debris removal. The enriched samples can then be used for a variety of downstream applications such as NGS, single-cell analysis, and compound testing.