As one of the most vital steps in sample preparation, processing tissue samples – such as resections and biopsies – is also the most challenging. Since these samples are often used for diagnostic and research purposes, it is critical to maintain cell population representation ratios from the original sample while ensuring the enrichment of target cells.
Additionally, because the enrichment and debris removal processes involve multiple complex steps that include exposing the sensitive cells to harsh physical and chemical conditions, low viability and yield are common outcomes. These challenges are a source of frustration for many scientists and researchers.
The LeviCellTM‘s groundbreaking levitation technology has proven to efficiently remove dead cells and debris while producing consistently higher yields and viabilities of fragile cell types from a variety of tissues, and has not resulted in changes in gene expression profile. The LeviCellTM platform enables the gentle selection of viable cells from tissues without the need for cell surface markers or antibodies, as these methods often lead to a biased selection, cell activation, and cell damage.
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A frozen vial of dissociated tumor cells (DTCs) was thawed in a water bath at 37ᵒC. With a small amount of ice remaining in the vial, the thawed cells were transferred into 10 mL of warmed media + 10% FBS dropwise. Cells were centrifuged at 500 rcf for 5 minutes. The supernatant was removed, and the pellet was resuspended in 10 mL of warm media. Cell count and viability were determined via Trypan blue staining at a 1:1 dilution.
Sample pipetted into the LeviCell™ cartridge.
Automated Label-Free Sorting
Precision density gradients drive levitation based on cells physical properties.
Selected populations collected into separate ports for further analysis or use.
The LeviCellTM Yield Difference
Unmatched Debris Removal
A list of tissue types tested on the LeviCellTM:
The LeviCellTM’s innovative levitation technology platform uses less than 1 psi of pressure to enable a completely touch-free, label-free, three-step gentle sorting process that takes only 20 minutes to complete. The system is truly a simple, fast, and reliable method for working with the complicated and traditionally difficult to process dissociated tumor samples for live-cell enrichment and debris removal. The enriched samples can then be used for a variety of downstream applications such as NGS, single-cell analysis, and compound testing.