Documents

Tech Note: New LeviCell Cartridge: Best Visualization and Same Superior Cell Enrichment

LevitasBio has built a solution for cellular enrichment and characterization around proprietary magnetic levitation technology. At its core, this technology is driven not only by the LeviCell instrument itself, but through its single-use consumable, the LeviCell cartridge. The new S2.3 cartridge design offers better visualization of levitating cells which enables powerful sample characterization through advanced software analytics while providing equivalent performance in sample enrichment.

Bring Your Cells Back to Life

PBMCs, special immune cells that are often the first line of defense in our bodies, are critical in a number of fields, including immunology, toxicology and precision medicine. Isolated PBMCs are often cryopreserved and stored until needed, presenting a common challenge – how can you ensure your cells are viable post-thaw?  Access this research snapshot to see how Levitation Technology can enrich for the most viable PBMCs from fresh and frozen samples.

One Simple Enrichment Step Takes scRNA-Seq to the Next Level

The quality of downstream scRNA-seq data and analysis can be degraded by poor quality samples.  Learn how a simple enrichment step can improve your QC metrics, allow you to retain 2.5 fold more cells, and decrease ambient RNA contamination by 1.7 fold.

Gentle Levitation Uncovers Native scRNA-Seq Transcriptomic Signatures

Isolation and interrogation of single cells from fresh brain tissue is crucial for scientists to understand their role in homeostasis, disease conditions and drug treatments. This application note illustrates how the LeviPrep-LeviCell workflow delivers enriched homeostatic microglia with 2-6 fold decrease in microglia activation markers than alternative isolation methods.

Simultaneous CD45+ Depletion and Viable Tumor Cell Enrichment

Access to all cells is important when studying the tumor microenvironment. However, characterization of genetic modulations in tumor cells often require removal of immune cells. Levitation Technology enables depletion of immune cells and simultaneous enrichment for viable tumor cells in one run.

Flow cytometry analysis can be confounded by low cell numbers, low cell viability and contaminants such as cellular debris. Sample optimization with levitation technology can address these challenges simultaneously.

Study of Disease Enhanced by Modulation of Levitation Height

While magnetic levitation alone can enrich for live cells from dead to dying cells, we demonstrate how the use of two cellular properties, density and magnetic susceptibility, can alter cells’ levitation height.

Poster: Rare CRISPR BCL-2 KO Cells Discovered Using Levitation Technology

Rare, surviving NALM-16 cells that had BCL-2 knocked out via CRISPR-mediated gene editing were successfully detected and enriched using the LeviCell system.

Parameters to Adjust when using Levicell for Viable Cell Enrichment

LeviCell technology together with its simple workflow, offers a good viable cell enrichment for most cells using the standard recommendations for levitation agent concentration (150 mM, final) and 20 min of levitation time.

Single Cell Enrichment Uncovers Results That Are Unaltered and Unbiased

The LeviCell maintains starting population heterogeneity and minimizes stress to maintain cell physiology, so you can have confidence in your cell preparations.

LeviCell vs. FACS: Increased Viability in Three Sample Enrichments Using Levitation

The groundbreaking LeviCell levitation technology efficiently removes dead cells and debris while producing high yields and viabilities of even fragile cell types from a variety of tissue types.

Simple and Robust Human Organoid Processing Using Levitation

As LeviCell technology provides a fast and easy separation of live and dead cells, it can be used to sort the resistant cells of an organoid culture after a drug treatment for further analysis and characterization.