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Tech Note: New LeviCell Cartridge: Best Visualization and Same Superior Cell Enrichment

LevitasBio has built a solution for cellular enrichment and characterization around proprietary magnetic levitation technology. At its core, this technology is driven not only by the LeviCell instrument itself, but through its single-use consumable, the LeviCell cartridge. The new S2.3 cartridge design offers better visualization of levitating cells which enables powerful sample characterization through advanced software analytics while providing equivalent performance in sample enrichment.

Enrichment of Viable Human PBMCs With Levitation Technology

PBMCs, special immune cells that are often the first line of defense in our bodies, are critical in a number of fields, including immunology, toxicology and precision medicine. Isolated PBMCs are often cryopreserved and stored until needed, presenting a common challenge – how can you ensure your cells are viable post-thaw?  Access this research snapshot to see how Levitation Technology can enrich for the most viable PBMCs from fresh and frozen samples.

Elevate the Quality of Your Single-Cell Sequencing Data Using Levitation Technology

The quality of downstream scRNA-seq data and analysis can be degraded by poor quality samples.  Learn how a simple enrichment step can improve your QC metrics, allow you to retain 2.5 fold more cells, and decrease ambient RNA contamination by 1.7 fold.

Enrichment of Homeostatic Mouse Brain Cell Subtypes Using Cold Dissociation and Gentle Levitation Technology

Isolation and interrogation of single cells from fresh brain tissue is crucial for scientists to understand their role in homeostasis, disease conditions and drug treatments. This application note illustrates how the LeviPrep-LeviCell workflow delivers enriched homeostatic microglia with 2-6 fold decrease in microglia activation markers than alternative isolation methods.

Preparation of Adult Mouse Brain for LeviCell System

These protocols provide guidance on how to work with both perfused and non-perfused adult mouse brain, and illustrates how one can enrich for either microglia only or all neural cell types using the LeviCell System.

Simultaneous CD45+ Depletion and Viable Tumor Cell Enrichment

Access to all cells is important when studying the tumor microenvironment. However, characterization of genetic modulations in tumor cells often require removal of immune cells. Levitation Technology enables depletion of immune cells and simultaneous enrichment for viable tumor cells in one run.

Clean-Up of Dissociated Tumor Cells (DTCs) Prior to Flow Cytometry Analysis

Flow cytometry analysis can be confounded by low cell numbers, low cell viability and contaminants such as cellular debris. Sample optimization with levitation technology can address these challenges simultaneously.

Leveraging Density and Magnetic Susceptibility to Modulate Levitation Height

While magnetic levitation alone can enrich for live cells from dead to dying cells, we demonstrate how the use of two cellular properties, density and magnetic susceptibility, can alter cells’ levitation height.

Poster: Rare CRISPR BCL-2 KO Cells Discovered Using Levitation Technology

Rare, surviving NALM-16 cells that had BCL-2 knocked out via CRISPR-mediated gene editing were successfully detected and enriched using the LeviCell system.

Parameters to Adjust when using Levicell for Viable Cell Enrichment

LeviCell technology together with its simple workflow, offers a good viable cell enrichment for most cells using the standard recommendations for levitation agent concentration (150 mM, final) and 20 min of levitation time.

Single Cell Analysis

The LeviCell maintains starting population heterogeneity and minimizes stress to maintain cell physiology, so you can have confidence in your cell preparations.

Tissue Processing

The groundbreaking LeviCell levitation technology efficiently removes dead cells and debris while producing high yields and viabilities of even fragile cell types from a variety of tissue types.

Organoids

As LeviCell technology provides a fast and easy separation of live and dead cells, it can be used to sort the resistant cells of an organoid culture after a drug treatment for further analysis and characterization.

Enriching for Human AVII from Tissue

To meet the need for high-viability isolation of sensitive and difficult cell types such as ATII cells, LevitasBio developed the LeviCell system – a powerful, label-free cell sorting platform to gently, rapidly, and efficiently separate and enrich cells.

Population Representation

The Innovative LeviCell Platform Removes Debris and Dead Cells While Maintaining Original Representation of Different Cellular Populations.

Stem Cells

The Promise of Stem Cell Therapies and How the LeviCell Can Contribute.

Large Cells and Organoid Processing

The LeviCell enables fast, easy, and robust sorting of large cells, organoids, and embryonic cells.

Adipocytes

The LeviCell is a gentle, label-free levitation technology which isolates and enriches a pure population of adipocytes in differentiated precursor cell models.

Cardiomyocytes

Since the demand for heart transplantation far exceeds the available supply, cell-based cardiac repair therapies are being aggressively pursued as a more pragmatic, cost-effective, and low-risk option. However, despite the decades-long studies performed on cardiomyocytes, this cell type remains one of the more challenging to isolate. The LeviCell offers a real and immediate solution.

Live vs. Bacterial Enrichment

The LeviCell System Establishes Highly Effective, Label- and Culture-Free, Robust Bacterial Enrichment, and Separation from Mammalian Cells.

Tissue Processing

The LeviCell Demonstrates Fast and Robust Live Cell Enrichment from a Range of Tissue Types.

Dissociated Tumor Cells

The LeviCell removes debris and enriches for live cells while preserving each unique cell type’s population ratio within the original tumor microenvironment.

LeviCell – Tech & Spec Sheet

The LeviCell platform is an all-in-one system designed to run the LeviCell disposable cartridge for label-free cell separation applications. The system includes control and analysis software and imaging.

Single Cell Sequencing

Rapid progress in the Single-cell analysis technologies field is necessary for the continued transformation of how we learn about and approach the clinical research, diagnosis, prognosis, and treatment of a wide range of disease types.

LeviCell System Enables Macrophage Research

As scientists and researchers working with macrophages in vitro know all too well, the promise of new and powerful insights in science that these key cell types offer come at a price of time, frustration, and challenges in reproducing results.

LeviCell – Workflow Comparison

See the advantages of the LeviCell system vs. BEADS or FACS.

Cell and Sample Types

Levitation technology has been extensively tested for a wide range of cell types and applications.

Peer-Reviewed Publication List

Find some of our most recent and interesting peer-reviewed publications describing the use of the technology for a variety of key applications.

Establishing Separation Parameters for Cell Separation Using Magnetic Levitation

Cell sorting and separation technologies are ubiquitous in molecular biology labs. Their popularity belies the serious limitations these tools have. For instance, flow cytometry requires antibody selection and therefore does not allow for a holistic look at a cellular population.

Levitas LeviCell Info Sheet

Download or print the latest spec sheet with platform advantages and specs.