Density gradient-based purification: rapid, simple and inexpensive

Oocytes are immature eggs that develop to maturity within the follicle. Granulosa cells (GC) provide the physical support and microenvironment required for the developing oocyte. These granulosa cells form a single layer around the oocytes in the primordial ovarian follicle and advance to form a multilayered cumulus oophorus surrounding the ovum in the follicle. The […]

Elevating Organoid Research with Levitation Technology

In 2009, Sato et al. study reported the establishment of the first 3D intestinal organoid culture derived from adult stem cells. Since then, many subsequent organoid protocols for other organs have appeared and are being widely used. These 3D in vitro cultures provide a powerful tool for a broad swath of research applications as they […]

Enhanced CRISPR-based Technology Speeds Identification of Genes Involved in Health and Disease

Today’s edition of the journal Science (August 19th; see “MIC-Drop: A platform for large-scale in vivo CRISPR screens”) reports a CRISPR-based method that can rapidly evaluate the functions of hundreds of genes in a single experiment while utilizing an animal model (Zebrafish) for the first time ever. Zebrafish are relatively fast-growing and share many of the same genes […]