Enriching for Differentiated Cardiomyocytes
With heart disease being one of the leading causes of death worldwide, deeper insight into the possible roles that cardiomyocytes play in the regeneration and repair of the heart is key to developing life-saving therapies. While surgical intervention (bypass, catheter, assist devices) continue to be the most widely implemented treatment, the nature of cardiovascular disease inevitably leads to heart failure – and subsequent need for a heart transplant.
Since the demand for heart transplantation far exceeds the available supply, cell-based cardiac repair therapies are being aggressively pursued as a more pragmatic, cost-effective, and low-risk option. However, despite the decades-long studies performed on cardiomyocytes, this cell type remains one of the more challenging to isolate. The LeviCellTM offers a real and immediate solution.
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Through its innovative levitation technology, the LeviCellTM harnesses unique properties of cardiomyocytes to isolate them from fibroblasts and enrich for differentiated live cardiomyocytes when working with stem cells. Since the LeviCellTM does not require dyes or antibodies, gene expression is not activated and population representation is preserved.
Additionally, the LeviCellTM platform’s 20-minute, three-step process efficiently separates cells without the need for repeated manual manipulation, enables purification of viable cardiomyocytes even with low starting numbers, and easily separates the target cell type from debris-filled samples.
Sample pipetted into the LeviCell™ cartridge.
Automated Label-Free Sorting
Precision density gradients drive levitation based on cells physical properties.
Selected populations collected into separate ports for further analysis or use.
Induced pluripotent stem cells (iPSCs) were differentiated into cardiomyocytes and metabolically starved before resuspending in levitation agent. Cardiomyocytes were enriched from the undifferentiated iPSCs and fibroblasts by processing with the LeviCellTM. Outputs were cultured in 6 well plates for 3-5 days to determine phenotype and viability. The separation on the LeviCellTM can be seen below in Fig. A
The density and resulting levitation height of the differentiated cardiomyoctyes is significantly higher then the non-cardiomyocyte population and can clearly seen in the density graph Fig. B and the enriched population vs. un-enriched in Fig. C.