Enriching for Differentiated Cardiomyocytes
Induced pluripotent stem cells (iPSCs) were differentiated into cardiomyocytes and metabolically starved before resuspending in levitation agent. Cardiomyocytes were enriched from the undifferentiated iPSCs and fibroblasts by processing with the LeviCellTM. Outputs were cultured in 6 well plates for 3-5 days to determine phenotype and viability. The separation on the LeviCellTM can be seen below in Fig. A
The density and resulting levitation height of the differentiated cardiomyoctyes is significantly higher then the non-cardiomyocyte population and can clearly seen in the density graph Fig. B and the enriched population vs. un-enriched in Fig. C.