The LeviCell™ System
Establishes Highly Effective, Label- and Culture-Free, Robust Bacterial Enrichment, and Separation from Mammalian Cells
The LeviCellTM’s three-step process eliminates the need for cell surface markers and antibodies – which often leads to preferential selection, cell damage, or cell activation. By simplifying the sample preparation procedure, the LeviCellTM reduces the risk for error while maximizing the potential success of downstream assays that include antibiotic resistance, food quality, water quality, and sepsis therapies.
Separation of Mammalian Cells from Bacteria
The following image shows the LeviCellTM platform’s demonstrated ability to efficiently and effectively separate bacterial cells from THP-1, a human monocytes cell line. Note the clear line of delineation. The collection of pure and viable E. coli cells for downstream assays increases the chances of successful subsequent analysis by eliminating the background of the mammalian cell population.
Separation of live vs dead Bacteria
In the following experiment, an E. coli sample was treated with gentamicin and mixed with an untreated sample to generate a mixed population, which was then loaded into the LeviCellTM. Live and dead populations are clearly observed levitating at different heights. The ability to test responses of different populations of bacteria to a variety of compounds without the need to culture and collect the resulting cells for downstream analysis is especially important when studying populations which are typically difficult – if not impossible – to culture.
Separation of Infected vs Uninfected Macrophages
In this example, the macrophage cell line RAW264.7 was infected with the bacterium Salmonella enterica. After infection, both the uninfected and the infected populations were fixed and loaded into the LeviCellTM for separation. The bulk of the cells post-infection clearly levitate higher than the uninfected cells, which simplifies the identification and collection of cells for downstream analysis.
Separation of infected vs. uninfected macrophages (Salmonella)