Undifferentiated precursor cells levitate at a uniform height, which indicates a homogeneous cell population. These cells were suspended in 100 mM Levitation Agent and allowed to reach their final levitation height shown in Figure 1b.
Cell Preparation for LeviCellTM
Different concentrations of Levitation Agent were used to determine which would provide maximum levitation distance between the two adipocyte cell populations.
Maximum levitation height difference between differentiated and undifferentiated adipocytes was achieved using 30 mM of Levitation Agent (Figure a above). Note that as the concentration of Levitation Agent increased, overall levitation height of both populations increased while the distance between differentiated and undifferentiated cells decreased
Starting samples for Fig c,d were differentiated for 7 days, indicating that the LeviCellTM can effectively isolate and enrich sensitive cells such as adipocytes after relatively early differentiation time points.
The LeviCellTM‘s demonstrated ability to efficiently and effectively isolate large, fragile adipocytes in a gentle, label-free, closed environment shows the platform’s immense potential to reset the bar in cell separation technology. Significant advances in understanding adipogenesis, adipocyte-related diseases, and fat tissue engineering are within reach as the LeviCellTM unambiguously exhibits its ability to enrich an abundance of pure and viable adipocytes in a shorter time span than conventional techniques.